Determination of tick positivity for pathogenic Borrelia burgdorferi by using PCR-RFLP method

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Authors

ŽÁKOVSKÁ Alena NEJEDLÁ Petra HOLÍKOVÁ Alena DENDIS Miloš

Year of publication 2004
Type Article in Periodical
Magazine / Source Scripta medica
MU Faculty or unit

Faculty of Science

Citation
Field Immunology
Keywords tick; Borrelia burgdorferi;Lyme disease; DFM; PCR; PCR-RFLP
Description The aim of the study was the monitoring of the presence of Borrelia burgdorferi in ticks Ixodes ricinus in the Pisárky park, Brno in 1996-2001. Ticks were analysed by DFM and next by the PCR method. The samples with a greater mass of borrelia DNA were analysed by the PCR-RFLP method and transfered into BSK-H medium and cultivated.
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