Addressing the role of vimentin in monocyte/macrophage differentiation using RNAi approach
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Article in Proceedings |
| Conference | RNA club |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | vimentin; differentiation; RNAi; phagocytosis |
| Description | Vimentin is one of the IF proteins that undergo significant changes both in expression, structure and subcellular location during maturation of hematopoietic cells. The v-myb-transformed monoblasts of the cell line BM2 can be induced to differentiate towards macrophage-like cells using phorbol ester TPA and histon deacetylase inhibitor trichostatin A (TSA). We described earlier that level of vimentin increases during TPA- or TSA-induced differentiation of BM2 cells. In this study, RNAi technology was used to explore the role of vimentin in differentiation of BM2 cells. The cells were transfected with vector expressing siRNA targeted to endogenous vimentin mRNA and several independ clones of stable transfectants exhibiting 70% reduction of cellular vimentin level were obtained. The lack of vimentin altered morphology of the cells, reduced their phagocytic activity and production of ROS upon treatment with TPA or TSA. Our results demonstrate that up-regulation of vimentin is essential for formation of fully active macrophages during terminal steps of monocyte/macrophage differentiation. |
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