Recognition of pore-forming colicin Y by its cognate immunity protein.

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Authors

ŠMAJS David MATĚJKOVÁ Petra WEINSTOCK G.M.

Year of publication 2006
Type Article in Periodical
Magazine / Source FEMS Microbiology Letters
MU Faculty or unit

Faculty of Medicine

Citation
Field Microbiology, virology
Keywords pore-forming colicin; colicin Y; colicin immunity; site-directed mutagenesis
Description Construction of hybrid immunity genes between colicin U (cui) and Y (cyi) immunity genes and site-directed mutagenesis of cyi were used to identify amino-acid residues of the colicin Y immunity protein (Cyi) involved in recognition of colicin Y. These amino-acid residues were localized close to the cytoplasmic site of the Cyi transmembrane helices T3 (S104, S107, F110, A112) and T4 (A159). Mutations in cui, which converted Cui sequence to Cyi sequence in positions 104, 107, 110, 112 and 159, resulted in an immunity gene that also conferred (besides immunity to colicin U) a high degree of immunity to colicin Y.
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