An immunohistochemical localization of SDF-1 and its receptor CXCR4 in dorsal root ganglia associated and non-associated to damaged nerve

Investor logo

Warning

This publication doesn't include Faculty of Education. It includes Faculty of Medicine. Official publication website can be found on muni.cz.
Authors

DUBOVÝ Petr KLUSÁKOVÁ Ilona SVÍŽENSKÁ Ivana JANČÁLEK Radim

Year of publication 2007
Type Appeared in Conference without Proceedings
MU Faculty or unit

Faculty of Medicine

Citation
Description The biological activity of stromal cell-derived factor (SDF1) is signaled through the chemokine receptor CXCR4. An immunohistochemical location of SDF-1 and CXCR4 proteins was investigated in the C7-C8 and L4-L5 dorsal root ganglia (DRG) of naive rats and those operated for unilateral L4-L5 spinal nerve ligature (SNL) and sciatic nerve ligature (ScNL). The naive DRG displayed immunofluorescence for SDF1-IF and CXCR4-IF at the surface of neurons and satellite glial cells (SGC). A significant decrease of SDF1-IF was induced in the ipsilateral, but remained distinct in the contralateral L4-L5 DRG one week after SNL. A bilateral reduction of SDF1-IF was found 2 weeks from SNL. A gradual diminution of SDF1-IF was observed bilaterally in the L4-L5 DRG neurons, but modest IF was in SGC after 1 and 2 weeks from ScNL, respectively. The SNL for 1 week induced a reduction of CXCR4-IF at the SGC surfaces of ipsi- in contrast to contralateral L4-L5 DRG, while intraneuronal staining was increased. Two weeks after SNL, the differences of CXCR4-IF at the neuronal surfaces in ipsilateral L4-L5 DRG and at the SGC surfaces in contralateral ones were marked. CXCR4-IF at the SGC surfaces remained in both ipsi- and contralateral DRG up to 2 weeks from ScNL, but it was increased intraneuronally only in the ipsilateral DRG. Similar pattern of SDF1- and CXCR4-IF was induced in the cervical DRG. A different expression of SDF1-IF and its receptor was observed in ipsi- and contralateral DRG associated and non-associated with unilateral SNL and ScNL. This suggests their different regulation with the time of survival and type of nerve injury.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.