Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays

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Authors

MATĚJKOVÁ Petra STROUHAL Michal ŠMAJS David NORRIS Steven J. PALZKILL Timothy PETROSINO Joseph F. SODERGREN Erica NORTON Jason E. SINGH Jaz RICHMOND Todd A. MOLLA Michael N. ALBERT Thomas J. WEINSTOCK George M.

Year of publication 2008
Type Article in Periodical
Magazine / Source BMC Microbiology
MU Faculty or unit

Faculty of Medicine

Citation
Web http://www.biomedcentral.com/1471-2180/8/76
Field Genetics and molecular biology
Keywords Treponema pallidum ssp. pallidum; strain SS14; oligonucleotide arrays
Description Syphilis spirochete Treponema pallidum ssp. pallidum remains the enigmatic pathogen, since no virulence factors have been identified and the pathogenesis of the disease is poorly understood. Increasing rates of new syphilis cases per year have been observed recently. The genome of the SS14 strain was sequenced to high accuracy by an oligonucleotide array strategy requiring hybridization to only three arrays (Comparative Genome Sequencing, CGS). Gaps in the resulting sequence were filled with targeted dideoxy-terminators (DDT) sequencing and the sequence was confirmed by whole genome fingerprinting (WGF). When compared to the Nichols strain, 327 single nucleotide substitutions (224 transitions, 103 transversions), 14 deletions, and 18 insertions were found. On the proteome level, the highest frequency of amino acid-altering substitution polymorphisms was in novel genes, while the lowest was in housekeeping genes, as expected by their evolutionary conservation. Evidence was also found for hypervariable regions and multiple regions showing intrastrain heterogeneity in the T. pallidum chromosome. The observed genetic changes do not have influence on the ability of Treponema pallidum to cause syphilitic infection, since both SS14 and Nichols are virulent in rabbit. However, this is the first assessment of the degree of variation between the two syphilis pathogens and paves the way for phylogenetic studies of this fascinating organism.
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