Identification of alphaB-crystallin, a biomarker of renal cell carcinoma by SELDI-TOF MS

Warning

This publication doesn't include Faculty of Education. It includes Faculty of Science. Official publication website can be found on muni.cz.

Authors	HOLČÁKOVÁ Jitka HERNYCHOVÁ Lenka BOUCHAL Pavel BROŽKOVÁ Kristýna ŽALOUDÍK Jan VALÍK Dalibor NENUTIL Rudolf VOJTĚŠEK Bořivoj
Year of publication	2008
Type	Article in Periodical
Magazine / Source	The International Journal of Biological Markers
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	alphaB-crystallin; biomarker; ProteinChip; renal cell carcionoma; SELDI-TOF MS
Description	Spectrometric-based surface-enhanced laser desorption/ionization ProteinChip (SELDI-TOF) facilitates rapid and easy analysis of protein mixtures and is often exploited to define potential diagnostic markers from sera. However, SELDI-TOF is a relatively insensitive technique and unable to detect circulating proteins at low levels even if they are differentially expressed in cancer patients. Therefore, we applied this technology to study tissues from renal cell carcinomas (RCC) in comparison to healthy controls. We found that different biomarkers are identified from tissues than those previously identified in serum, and that serum markers are often not produced by the tumors themselves at detectable levels, reflecting the nonspecific nature of many circulating biomarkers. We detected and characterized alphaB-crystallin as an overexpressed protein in RCC tissues and showed differential expression by immunohistochemistry.
Related projects:	Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine