Ten new temperate bacteriophages of Citrobacter youngae.

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Authors

ŠMARDA Jan SLOVÁČKOVÁ H.

Year of publication 2004
Type Article in Periodical
Magazine / Source Folia microbiologica
MU Faculty or unit

Faculty of Medicine

Citation
Web http://www.cssm.info/priloha/fm2004_671.pdf
Field Microbiology, virology
Keywords DNA HYBRIDIZATION; SP-NOV; CLASSIFICATION; BACTERIOCIN; STRAINS; PHAGES
Description In a cross-test, we examined 55 strains of Citrobacter youngae against each other as potential producers of temperate bacteriophages and as potential sensitive indicators for them. Ten strains (18.2%) showed the production of phages. Seven different strain-specific spectra of activity (from 1 to 11 strains each) were found. Phage production by 6 strains was inducible with mitomycin C, in 4 strains it was not inducible. Using a polyclonal, specific anti-lambda serum, all 10 phages were found to be clearly distinct from E. coli lambda phage, the phage 31/47 showing the highest neutralization titre of all. Five phage-immune clones lysogenized with 5 of the phages kept their remaining phage sensitivity spectra, though extended by sensitivity to 1-3 phages.The phages belong to the morphotypes of Myoviridae (6 phages) and Siphoviridae (4 phages), with head diameters of 51-58 nm and tail length of 97-173 nm. Three strains produced corpuscular bacteriocins.
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