RETENTION BEHAVIOUR OF OLIGONUCLEOTIDES ISOMERS STUDIED BY MEANS OF HYDROPHILIC INTERACTION CHROMATOGRAPHY

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Authors

FUKSOVÁ Hana HÉGROVÁ Blanka KOSTEČKA Pavel TRNKOVÁ Libuše BITTOVÁ Miroslava

Year of publication 2010
Type Article in Proceedings
Conference X. pracovní setkání fyzikálních chemiků a elektrochemiků - sborník příspěvků
MU Faculty or unit

Faculty of Science

Citation
Field Analytic chemistry
Keywords oligonucleotides; chromatography
Description Short oligonucleotides and its isomers are deeply studied for better understanding of nucleic acid properties and interactions. High performance liquid chromatography (HPLC) provides suitable tool for their characterization. Our interest in this study was focused on the separation of pentamers, consisted of adenine, guanine and thymine by means of hydrophilic liquid chromatography mode (HILIC). The influence of experimental parameters such as mobile phase pH and/or buffer concentration, percentage of organic solvent in mobile phase and flow rate was evaluated and the most acceptable conditions were selected for further analysis. Finally, obtained results were compared with commonly applied reverse phase liquid chromatography (RP HPLC).
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