Spectroscopic study of protonation of cytosine-containing oligonucleotides

Warning

This publication doesn't include Faculty of Education. It includes Faculty of Science. Official publication website can be found on muni.cz.

Authors	HOLUBOVÁ Sylvie LUBAL Přemysl FARKOVÁ Marta TRNKOVÁ Libuše HÉGROVÁ Blanka KRISTOVÁ Petra
Year of publication	2010
Type	Article in Proceedings
Conference	Sborníi 14. česko - slovenské spektroskopické konference
MU Faculty or unit	Faculty of Science
Citation
Field	Analytic chemistry
Keywords	spectroscopy; cytosine; protonation constant
Description	Cytosine is an important building block of nucleic acids taking part in the process of genetic information transfer and transfer of energy of biologically important compounds by means of coenzymes. Due to acidobasic properties of bioligands, the presence of protonated species is dependent on the protonation constants. It was also shown that oligonucleotides (ODNs) concerning cytosine can form the so called structural i motif as a consequence of probable stabilization of the secondary structure of biomacromolecules. The aim of this work was to determine the protonation constants of ODNs varying the number of bases in order to see their influence on thermodynamic stability of studied ligands.
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine