Selective isolation of DNA from food matrices

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Authors

ŠPANOVÁ Alena TVRDÍKOVÁ Jana HORÁK Daniel RITTICH Bohuslav

Year of publication 2010
Type Article in Proceedings
Conference 30th International Symposium on the Separation of Proteins, Peptides and Polynucleotides
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords magnetic particles; streptavidin; Lactobacillus DNA separation; PCR
Description Research has been focused on the development of innovative magnetic micrometer-sized microspheres for specific applications in DNA analysis in food matrices and other real samples. Selective isolation of high-quality DNA can be performed using suitable functionalised magnetic microspheres. Magnetic P(HEMA-co-GMA) microspheres functionalised with streptavidin were used for immobilisation of biotinylated DNA probes (primers, PCR products) and for selective DNA isolation of Lactobacillus paracasei using DNA/DNA hybridisation. The procedure proposed was applied for target isolation of DNA from probiotic bacteria in complex samples (probiotic food supplements). Isolated DNA was used in genus- and species-specific PCRs.
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