Reversible DNA adsorption on the surfaces of different magnetic nano- and microspheres.
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Article in Proceedings |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | magnetic hydrophilic microspheres; P(HEMA-co-GMA); perovskites nanoparticles; PCR-ready DNA; PCR |
| Description | Non-selective isolation of high-quality DNA can be performed using carboxyl group-containing magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres. This procedure was used for PCR-ready DNA isolation and identification of Lactobacillus, Bifidobacterium and psychrotrophic bacteria in different dairy products or sediments. Analogically the magnetic microparticles P(HEMA-co-GMA) functionalised with streptavidin were used for immobilisation of biotinylated DNA probe and for selective isolation of target DNA from complex samples (e.g. food supplements) using DNA/DNA hybridisation as well as lanthanum manganese perovskites nanoparticles encapsulated into a thin silica shell. |
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