Reversible DNA adsorption on the surfaces of different magnetic nano- and microspheres.

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Authors

ŠPANOVÁ Alena TVRDÍKOVÁ Jana HORÁK Daniel KAMAN Ondřej POLLERT Emil RITTICH Bohuslav

Year of publication 2010
Type Article in Proceedings
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords magnetic hydrophilic microspheres; P(HEMA-co-GMA); perovskites nanoparticles; PCR-ready DNA; PCR
Description Non-selective isolation of high-quality DNA can be performed using carboxyl group-containing magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres. This procedure was used for PCR-ready DNA isolation and identification of Lactobacillus, Bifidobacterium and psychrotrophic bacteria in different dairy products or sediments. Analogically the magnetic microparticles P(HEMA-co-GMA) functionalised with streptavidin were used for immobilisation of biotinylated DNA probe and for selective isolation of target DNA from complex samples (e.g. food supplements) using DNA/DNA hybridisation as well as lanthanum manganese perovskites nanoparticles encapsulated into a thin silica shell.
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