SPECTROPHOTOMETRIC ACID-BASE CHARACTERIZATION OF ADENINE ANALOGUES
| Authors | |
|---|---|
| Year of publication | 2011 |
| Type | Article in Proceedings |
| Conference | XI. Pracovní setkání fyzikálních chemiků a elektrochemiků |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | purine based;RNA;DNA;adenine;guanine;aminopurines;acid-based properties;UV-Vis spectroscopy;fluorescence |
| Description | Purine based DNA/RNA bases adenine (6-aminopurine) and guanine (2-aminopurin-6- one), serve in live bodies as building blocks of high physiological importance. For in vitro research applications they are replaced by 2-aminopurine and 2,6-diaminopurine. These aminopurines are used either “free” as replacements for adenine/guanine in nucleic acid strands or are subject to further derivatization and use (e.g. enzyme function research [1]). Despite their frequent use, very few is known about the substances’ physico-chemical properties. We studied acid-base properties of 2-aminopurine and 2,6-diaminopurine with use of advanced chemometric tools applied to UV/Vis spectroscopy data and since the 2- aminopurine is highly fluorescent, fluorescence spectroscopy data, too. |
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