Investigation of Matrix Deposition Techniques for High Throughput MALDI MS

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Authors	BEDNAŘÍK Antonín ERTL Lukáš RÁŽOVÁ Iva TOMALOVÁ Iva PREISLER Jan MOSKOVETS Eugene
Year of publication	2011
Type	Conference abstract
MU Faculty or unit	Central European Institute of Technology
Citation
Description	Matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is an emerging technique with an immense biochemical and pharmaceutical potential, which possesses ability to show spatial distribution of specific compounds in tissues. The crucial step of MALDI-MSI experiment is matrix deposition. The MALDI matrix together with rhodamine B labeled angiotensin I was deposited on sample MALDI plate by three different approaches. By electrospray, spin-coating and piezoelectric nano-spotter. The homogeneity of deposited samples was studied under microscope by observing simple optical image and by observing fluorescence image after irradiating the sample with 532 nm laser. Optical image was compared with the MSI generated map of peptide distribution along the samples. To measure spatial distribution of selected peptides, a laboratory-built high-throughput axial time-of-flight (TOF) mass spectrometer with 2 kHz 355 nm Nd:YAG laser was used.
Related projects:	Molecular basis of cell and tissue regulations Analýza biomolekul hmotnostní spektrometrií s laserovou desorpcí/ionizací za účasti nanomateriálů CEITEC - Central European Institute of Technology