Classification of Pseudomonas spp. isolated from Antarctic samples
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| Rok publikování | 2012 |
| Druh | Další prezentace na konferencích |
| Citace | |
| Popis | It is well established that several bacterial species inhabit extremely cold environments. Our study was focused on Pseudomonas bacteria isolated from numerous samples of rock particles, small stones, soil crusts and surface layers collected in deglaciated terrestrial ecosystems of the Northern part of James Ross Island, Antarctica (63 48 02 S, 57 52 57 W) within the period of 2007-2010. Typical sites of sample collection were shaded from direct sunshine by large stones or rock walls. Microhabitats were either vegetation free or with sparse moss and lichen vegetation. Genotypic and phenotypic tools were used to characterize overall 146 isolated fluorescent strains of Gram negative rods. Genotypic methods comprised of manual ribotyping with HindIII endonuclease, followed by multilocus sequence analyses of rrs, rpoB and rpoD genes. The analyses of phenotype involved extensive biotyping, protein profiling using SDS-PAGE, and MALDI-TOF MS. Some isolates formed separate clusters in protein and ribotype fingerprintings which were represented by the strains of different origin. We confirmed the presence of identical or closely related strains of the same Pseudomonas species isolated from different samples. Our recent studies have led to characterize a few of novel species (e.g. „Pseudomonas prosekii"). Moreover, the results of ribotyping indicated probably more novel species among isolated fluorescent strains. Therefore, further analyses will be carried out to characterize bacterial diversity of this psychrotrophic pseudomonads from various Antarctic samples. This work was partly supported by projects from the Ministry of Education, Youth and Sports of the Czech Republic (MSM0021622415, MSM0021622416 and LM2010009). |
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