Bacterial community dynamics in a rumen fluid bioreactor during in-vitro cultivation

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Publikace nespadá pod Pedagogickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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ZAPLETALOVÁ Martina KAŠPAROVSKÁ Jitka KŘÍŽOVÁ Ludmila KAŠPAROVSKÝ Tomáš ŠERÝ Omar LOCHMAN Jan

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Biotechnology
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Doi http://dx.doi.org/10.1016/j.jbiotec.2016.07.013
Obor Biochemie
Klíčová slova Rumen fluid; Bioreactor; Redox potential; Microbiome analysis; 16S rDNA
Popis To study the various processes in the rumen the in vitro techniques are widely used to realize more controlled and reproducible conditions compared to in vivo experiments. Mostly, only the parameters like pH changes, volatile fatty acids content or metabolite production are monitored. In this study we examine the bacterial community dynamics of rumen fluid in course of ten day cultivation realize under standard conditions described in the literature. Whereas the pH values, total VFA content and A/P ratio in bioreactor were consistent with natural conditions in the rumen, the mean redox-potential values of 251 and 243 mV were much more negative. For culture-independent assessment of bacterial community composition, the Illumina MiSeq results indicated that the community contained 292 bacterial genera. In course of ten days cultivation a significant changes in the microbial community were measured when Bacteroidetes to Firmicutes ratio changed from 3.2 to 1.2 and phyla Proteo bacteria and Actino bacteria represented by genus Bifido bacterium and Olsenella significantly increased. The main responsible factor of these changes seems to be very low redox potential in bioreactor together with accumulation of simple carbohydrates in milieu as a result of limited excretion of fermented feed and absence of nutrient absorbing mechanisms. (C) 2016 Elsevier B.V. All rights reserved.
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