Cell-surface receptors enable perception of extracellular cytokinins

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Publikace nespadá pod Pedagogickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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ANTONIADI Ioanna NOVÁK Ondřej GELOVÁ Zuzana JOHNSON Alexander PLÍHAL Ondřej SIMERSKÝ Radim MIK Václav VAIN Thomas MATEO-BONMATÍ Eduardo KARADY Michal PERNISOVÁ Markéta PLAČKOVÁ Lenka OPASSATHIAN Korawit HEJÁTKO Jan ROBERT Stéphanie FRIML Jiří DOLEŽAL Karel LJUNG Karin TURNBULL Colin

Rok publikování 2020
Druh Článek v odborném periodiku
Časopis / Zdroj NATURE COMMUNICATIONS
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://www.nature.com/articles/s41467-020-17700-9
Doi http://dx.doi.org/10.1038/s41467-020-17700-9
Klíčová slova cytokinin signalling
Popis Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors are substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception and importance of spatially heterogeneous cytokinin distribution continue to be debated. Here we show that cytokinin perception by plasma membrane receptors is an effective additional path for cytokinin response. Readout from a Two Component Signalling cytokinin-specific reporter (TCSn::GFP) closely matches intracellular cytokinin content in roots, yet we also find cytokinins in extracellular fluid, potentially enabling action at the cell surface. Cytokinins covalently linked to beads that could not pass the plasma membrane increased expression of both TCSn::GFP and Cytokinin Response Factors. Super-resolution microscopy of GFP-labelled receptors and diminished TCSn::GFP response to immobilised cytokinins in cytokinin receptor mutants, further indicate that receptors can function at the cell surface. We argue that dual intracellular and surface locations may augment flexibility of cytokinin responses.
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