Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration

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Publikace nespadá pod Pedagogickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
Název česky Účast terminální oxidasy typu cbb(3) při růstové kompetici bakterií, tvorbě biofilmu a přepnutí mezi denitrifikací a aerobní respirací
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KUČERA Igor SEDLÁČEK Vojtěch

Rok publikování 2020
Druh Článek v odborném periodiku
Časopis / Zdroj Microorganisms
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://doi.org/10.3390/microorganisms8081230
Doi http://dx.doi.org/10.3390/microorganisms8081230
Klíčová slova respiratory chain; terminal oxidases; denitrification; branched electron flow; biofilm
Popis Paracoccus denitrificans has a branched electron transport chain with three terminal oxidases transferring electrons to molecular oxygen, namely aa(3)-type and cbb(3)-type cytochrome c oxidases and ba(3)-type ubiquinol oxidase. In the present study, we focused on strains expressing only one of these enzymes. The competition experiments showed that possession of cbb(3)-type oxidase confers significant fitness advantage during oxygen-limited growth and supports the biofilm lifestyle. The aa(3)-type oxidase was shown to allow rapid aerobic growth at a high oxygen supply. Activity of the denitrification pathway that had been expressed in cells grown anaerobically with nitrate was fully inhibitable by oxygen only in wild-type and cbb(3) strains, while in strains aa(3) and ba(3) dinitrogen production from nitrate and oxygen consumption occurred simultaneously. Together, the results highlight the importance of the cbb(3)-type oxidase for the denitrification phenotype and suggest a way of obtaining novel bacterial strains capable of aerobic denitrification.
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