In Vitro Characterization of Sumoylation of HR Proteins

Varování

Publikace nespadá pod Pedagogickou fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	ALTMANNOVÁ Veronika KREJČÍ Lumír
Rok publikování	2021
Druh	Kapitola v knize
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
Popis	In vitro analysis of posttranslational modifications such as sumoylation provides a great tool to not only identify the target proteins but also to characterize the specific effects of this modification on the protein features and uncover possible regulatory mechanism. In this chapter, we will describe the purification of yeast SUMO machinery proteins and their use to identify SUMO modification of target proteins in vitro. Furthermore, we will show several examples characterizing the effect of sumoylation on the biochemical activities of various proteins involved in homologous recombination (HR) that helped to better understand the regulatory role of this modification.
Související projekty:	Vnitřní vlastnosti RAD51 vlákna a jeho biologické regulace Mechanics and execution of homologous recombination - biophysics to the organism Preklinická progrese nových organických sloučenin s cílenou biologickou aktivitou