Infrared Laser Desorption of Intact Nanoparticles for Digital Tissue Imaging

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Publikace nespadá pod Pedagogickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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STIBOREK Marek JINDŘICHOVÁ Lenka MELIORISOVÁ Stanislava BEDNAŘÍK Antonín PRYSIAZHNYI Vadym KROUPA Jiří HOUŠKA Pavel ADAMOVÁ Barbora NAVRÁTILOVÁ Jarmila KANICKÝ Viktor PREISLER Jan

Rok publikování 2022
Druh Článek v odborném periodiku
Časopis / Zdroj Analytical Chemistry
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://pubs.acs.org/doi/full/10.1021/acs.analchem.2c05216
Doi http://dx.doi.org/10.1021/acs.analchem.2c05216
Klíčová slova Ablation; Biopolymers; Gold; Lasers; Metal nanoparticles
Přiložené soubory
Popis We report a new technique for the digital mapping of biomarkers in tissues based on desorption and counting intact gold nanoparticle (Au NP) tags using infrared laser ablation single-particle inductively coupled plasma mass spectrometry (IR LA SP ICP MS). In contrast to conventional UV laser ablation, Au NPs are not disintegrated during the desorption process due to their low absorption at 2940 nm. A mass spectrometer detects up to 83% of Au NPs. The technique is demonstrated on mapping a proliferation marker, nuclear protein Ki-67, in three-dimensional (3D) aggregates of colorectal carcinoma cells, and the results are compared with confocal fluorescence microscopy and UV LA ICP MS. Precise counting of 20 nm Au NPs with a single-particle detection limit in each pixel by the new approach generates sharp distribution maps of a specific biomarker in the tissue. Advantageously, the desorption of Au NPs from regions outside the tissue is strongly suppressed. The developed methodology promises multiplex mapping of low-abundant biomarkers in numerous biological and medical applications using multielemental mass spectrometers.
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