Small RNA Sequencing Identifies a Six-MicroRNA Signature Enabling Classification of Brain Metastases According to their Origin

Logo poskytovatele
Logo poskytovatele

Varování

Publikace nespadá pod Pedagogickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
Autoři

ROŠKOVÁ Ivana VEČEŘA Marek RADOVÁ Lenka TRACHTOVÁ Karolína SIEGL František HERMANOVÁ Markéta HENDRYCH Michal KŘEN Leoš VYBÍHAL Václav VALEKOVÁ Hana KASPAROVA Petra KOLOUŠKOVÁ Ivana KAZDA Tomáš SLABÝ Ondřej JANČÁLEK Radim ŠÁNA Jiří SMRČKA Martin

Rok publikování 2023
Druh Článek v odborném periodiku
Časopis / Zdroj CANCER GENOMICS & PROTEOMICS
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://cgp.iiarjournals.org/content/20/1/18
Doi http://dx.doi.org/10.21873/cgp.20361
Klíčová slova Brain metastases; microRNA; small RNA sequencing; classifier; diagnosis
Přiložené soubory
Popis Background/Aim: Brain metastases (BMs) are the most frequent intracranial tumors in adults and one of the greatest challenges for modern oncology. Most are derived from lung, breast, renal cell, and colorectal carcinomas and melanomas. Up to 14% of patients are diagnosed with BMs of unknown primary, which are commonly characterized by an early and aggressive metastatic spread. It is important to discover novel biomarkers for early identification of BM origin, allowing better management of patients with this disease. Our study focused on microRNAs (miRNAs), which are very stable in frozen native and FFPE tissues and have been shown to be sensitive and specific diagnostic biomarkers of cancer. We aimed to identify miRNAs with significantly different expression in the five most frequent groups of BMs and develop a diagnostic classifier capable of sensitive and specific classification of BMs. Materials and Methods: Total RNA enriched for miRNAs was isolated using the mirVana miRNA Isolation Kit from 71 fresh-frozen histopathologically confirmed BM tissues originating in 5 cancer types. Sequencing libraries were prepared using the QIAseq miRNA Library Kit and sequenced on the NextSeq 500 platform. MiRNA expression was further validated by RT-qPCR. Results: Differential analysis identified 373 miRNAs with significantly different expression between 5 BM groups (p<0.001). A classifier model was developed based on the expression of 6 miRNAs (hsa-miR-141-3p, hsa-miR-141-5p, hsa-miR-146a-5p, hsa-miR-194-5p, hsa-miR-200b-3p and hsa-miR-365b-5p) with the ability to correctly classify 91.5% of samples. Subsequent validation confirmed both significantly different expression of selected miRNAs in 5 BM groups as well as their diagnostic potential. Conclusion: To date, our study is the first to analyze miRNA expression in various types of BMs using small RNA sequencing to develop a diagnostic classifier and, thus, to help stratify BMs of unknown primary. The presented results confirm the importance of studying the dysregulated expression of miRNAs in BMs and the diagnostic potential of the validated 6-miRNA signature.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.