Spatial distribution of centromeric and subtelomeric DNA sequences in interphase nuclei of human Go and stimulated lymphocytes

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Publikace nespadá pod Pedagogickou fakultu, ale pod Fakultu informatiky. Oficiální stránka publikace je na webu muni.cz.
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AMRICHOVÁ Jana LUKÁŠOVÁ Emilie KOZUBEK Stanislav JIRSOVÁ Pavla KOZUBEK Michal

Rok publikování 2002
Druh Článek ve sborníku
Konference Biophysics of the genome and its interactions
Fakulta / Pracoviště MU

Fakulta informatiky

Citace
Obor Genetika a molekulární biologie
Klíčová slova telomere; centromere; interpahse nuclei; chromatin structure
Popis Nuclear location of subtelomeric and centromeric DNA sequences of chromosome 8, 9 and 13 was investigated in 3D fixed human G0 and stimulated T-lymphocytes using repeated dual colour FISH. In the first hybridisation, DNA probes for p and q arms labelled by different fluorochromes were used. The centromere and the chromosome territory of the same chromosome were visualised in the second hybridisation on the same nuclei. It was found for all investigated chromosomes that contrary to centromeres located in the proximity of the nuclear membrane, the telomeres adopt more central position. These results indicate a hairpin like structure of chromosomes in the interphase nucleus. The results of this study provide information not only on the topography of subtelomeres but also images of the spatial arrangement and orientation of interphase chromosomes in cycling and non-cycling cell nuclei.
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