Image Division Technique in Pre-acquisition Analysis of Information Content for Automated Microscopy

Varování

Publikace nespadá pod Pedagogickou fakultu, ale pod Fakultu informatiky. Oficiální stránka publikace je na webu muni.cz.
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BRÁZDILOVÁ Silvie Luisa KOZUBEK Michal

Rok publikování 2011
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Microscopy
Fakulta / Pracoviště MU

Fakulta informatiky

Citace
Doi http://dx.doi.org/10.1111/j.1365-2818.2010.03465.x
Obor Využití počítačů, robotika a její aplikace
Klíčová slova Automated microscopy; fluorescence microscopy; autofocusing; multimodal content function; image acquisition; information content analysis
Popis This article presents a method that allows for reliable automated image acquisition of specimens with high information content in light microscopy with emphasis on fluorescence microscopy applications. Automated microscopy typically relies on autofocusing used for the analysis of information content behaviour along z-axis within each field of view. However, in the case of a field of view containing more objects that do not lie precisely in one z-plane, traditional autofocusing methods fail due to their principle of operation. We avoid this issue by reducing the original problem to a set of simple and performable tasks: we divide the field of view into a small number of tiles and process each of them individually. The obtained results enable discovering z-planes with rich information content that remain hidden during global analysis of the whole field of view. Our approach therefore outperforms other acquisition methods including the manual one. A large part of the contribution is oriented toward practical application.
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