Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver.

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Publikace nespadá pod Pedagogickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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TRNKOVÁ Libuše KRIZKOVA Soňa ADAM Vojtěch HUBÁLEK Jaromír KIZEK René

Rok publikování 2011
Druh Článek v odborném periodiku
Časopis / Zdroj BIOSENSORS & BIOELECTRONICS
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://ac.els-cdn.com/S0956566310006494/1-s2.0-S0956566310006494-main.pdf?_tid=45582c491f72810960ab2a3968016b45&acdnat=1332250805_449357aac5e95feac50ebc201b6b9175
Doi http://dx.doi.org/10.1016/j.bios.2010.09.035
Obor Fyzikální chemie a teoretická chemie
Klíčová slova Heavy metal; Silver; Metallothionein; Square wave voltammetry; Water; Environmental analysis
Popis In this paper, heavy metal biosensor based on immobilization of metallothionein (MT) to the surface of carbon paste electrode (CPE) via anti-MT-antibodies is reported. First, the evaluation of MT electroactivity was done. The attention was focused on the capturing of MT to the CPE surface. Antibodies incorporated and mixed into carbon paste were stable; even after two weeks the observed changes in signal height were lower than 5%. Further, the interaction of MT with polyclonal chicken antibodies incorporated in carbon paste electrode was determined by square-wave voltammetry. In the voltammogram, two signals - labelled as cys(MT) and W(a) - were observed. The cyst,AT corresponded to -SH moieties of MT and W(a) corresponded to tryptophan residues of chicken antibodies. Time of interaction (300 s) and MT concentration (125 mu g/ml) were optimized to suggest a silver(I) ions biosensor. Biosensor (CPE modified with anti-MT antibody) prepared under the optimized conditions was then used for silver(I) ions detection. The detection limit (3 S/N) for silver(I) ions was estimated as 0.5 nM. The proposed biosensor was tested by detection spiking of silver(I) ions in various water samples (from very pure distilled water to rainwater). Recoveries varied from 74 to 104%. (C) 2010 Elsevier B.V. All rights reserved.
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