Informace o projektu
Dynamics of telomeres and rDNA loci in plant cell nuclei
- Kód projektu
- LTC20003
- Období řešení
- 6/2020 - 4/2023
- Investor / Programový rámec / typ projektu
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Ministerstvo školství, mládeže a tělovýchovy ČR
- INTER-EXCELLENCE
- INTER-COST
- Fakulta / Pracoviště MU
- Středoevropský technologický institut
The cell nucleus and its detailed structure has always represented an exciting cellular compartment and stimulated establishment of the new research areas and innovative methodologies. Plants represent important model systems with a high developmental plasticity and variable organization of their genomes, differing, e.g., in the genome size, proportion of repetitive elements or the chromosome numbers. Our research group integrates studies in the field of telomere biology, chromatin structure, DNA repair and epigenetics. Rapid progress of the cell biology occurred in line with development of super-resolution microscopy approaches and advanced assays to investigate chromatin organization, allowing a detailed insight into the spatial organization of individual nuclear subdomains and other functional elements, such as nucleolus, telomeres or clusters of ribosomal rRNA genes. In the proposed project, we will investigate the effect of molecular and environmental factors on telomere composition and telomerase regulation and decipher functional links between chromatin assembly and epigenetic memory in the telomere maintenance, representing a good measure of overall genome stability.
Cíle udržitelného rozvoje
Masarykova univerzita se hlásí k cílům udržitelného rozvoje OSN, jejichž záměrem je do roku 2030 zlepšit podmínky a kvalitu života na naší planetě.
Publikace
Počet publikací: 13
2023
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Histone H1 protects telomeric repeats from H3K27me3 invasion in Arabidopsis
Cell Reports, rok: 2023, ročník: 42, vydání: 8, DOI
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Identification of the Sequence and the Length of Telomere DNA
Plant Cytogenetics and Cytogenomics : Plant Cytogenetics and Cytogenomics, rok: 2023, počet stran: 18 s.
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Sulfate supplementation affects nutrient and photosynthetic status of Arabidopsis thaliana and Nicotiana tabacum differently under prolonged exposure to cadmium
Journal of Hazardous Materials, rok: 2023, ročník: 445, vydání: March, DOI
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Visualization of the Nucleolus Using 5′ Ethynyl Uridine
Methods in Molecular Biology, volume 2672, rok: 2023, počet stran: 15 s.
2022
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Laser microirradiation as a versatile system for probing protein recruitment and protein-protein interactions at DNA lesions in plants
New Phytologist, rok: 2022, ročník: 234, vydání: 5, DOI
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MEDIATOR SUBUNIT17 integrates jasmonate and auxin signaling pathways to regulate thermomorphogenesis
Plant Physiology, rok: 2022, ročník: 189, vydání: 4, DOI
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Telomerase Interaction Partners-Insight from Plants
International Journal of Molecular Sciences, rok: 2022, ročník: 23, vydání: 1, DOI
2021
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Distinct Responses of Arabidopsis Telomeres and Transposable Elements to Zebularine Exposure
International Journal of Molecular Sciences, rok: 2021, ročník: 22, vydání: 1, DOI
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G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability
Plant Physiology, rok: 2021, ročník: 186, vydání: 4, DOI
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Super-resolution microscopy of chromatin fibers and quantitative DNA methylation analysis of DNA fiber preparations
Journal of Cell Science, rok: 2021, ročník: 134, vydání: 15, DOI