ATP measurements in iron-oxidizing Acidithiobacillus ferrooxidans

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Authors

MANDL Martin MARKOVÁ Romana LOJEK Antonín

Year of publication 2009
Type Article in Periodical
Magazine / Source Advanced Materials Research
MU Faculty or unit

Faculty of Science

Citation
Field Mining industry incl. coal mining and processing
Keywords Acidithiobacillus ferrooxidans; ATP; iron-oxidizing bacteria
Description We applied a kit for bioluminescent ATP measurement based on the luciferase reaction to Acidithiobacillus ferrooxidans cultures. Determination limit for monitoring cell growth in the culture was 1 . 106 cells per ml. Determination errors varied between 1.5 and 10 % (RSD). ATP formation correlated with cell growth and iron oxidation up to limitation by iron(II). A maximum ATP content was approximately 1 amol per cell under the growth yield on iron of 1 . 1012 cells per mol Fe(II).
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