CRACKING THE LECTIN CODE ; IN SILICO MODELING AND STRUCTURE-FUNCTIONAL STUDY OF PRINCIPLES DRIVING SUGAR PREFERENCE IN PAIIL FAMILY
| Authors | |
|---|---|
| Year of publication | 2009 |
| Type | Article in Proceedings |
| Conference | FEBS Journal |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | lectin engineering; molecular modeling; thermodynamics |
| Description | INTRODUCTION Pseudomonas aeruginosa is an opportunistic human pathogen, a bacterium capable of attacking individuals with lowered immunity barriers. It is e.g. responsible for lethal complications in patients with cystic fibrosis. The PAIIL lectin (a C type fucose-preferring lectin with sugar binding mediated by two calcium ions), produced by the bacterium plays a crucial role in the host pathogen interaction. Similar lectin sequences were found in other bacteria, displaying distinct differences in preference despite only small differences in structure of binding site. In vitro and in silico mutants were constructed in order to analyze the principles driving the sugar preference. METHODS Molecular docking was performed using the AUTODOCK and DOCK software. |
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