Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen
| Authors | |
|---|---|
| Year of publication | 2011 |
| Type | Article in Periodical |
| Magazine / Source | Folia Microbiologica |
| MU Faculty or unit | |
| Citation | |
| Web | http://www.springerlink.com/content/u876462762641134/ |
| Doi | http://dx.doi.org/10.1007/s12223-011-0004-6 |
| Field | Biochemistry |
| Keywords | nitrate reductase; artificial electron donor; nitrate transport; inhibition |
| Description | A comparative examination of reduced methyl [MV](+) and benzyl [BV](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activity with [MV](+) was high in whole-cell assays, confirming that this compound donates electrons to Nar. Initial rates of the more lipophilic [BV](+) were considerably lower, which was interpreted to be caused by an inhibition of the active transport of nitrate into the cells. Anionophoric activity of [BV](+) was detectable but too low to effectively circumvent the inhibition of nitrate transporter. |
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