Analysis of GFP and AHP5-GFP Fusion Protein in the Plant Extract Using CE-LIF
| Authors | |
|---|---|
| Year of publication | 2012 |
| Type | Article in Proceedings |
| Conference | CECE 2012 9th International Interdisciplinary Meeting on Bioanalysis |
| MU Faculty or unit | |
| Citation | |
| Web | http://www.ce-ce.org/cece2012/Proceedings%20CECE%202012%20Final+late%20abstracts_web.pdf |
| Field | Biochemistry |
| Keywords | CE-LIF; GF; fusion protein AHP5-GFP |
| Description | We focused on the possibility of determination green fluorescent protein (GFP) and Arabidopsis histidine phophotransfer protein 5 fused with GFP (AHP5-GFP) in complex plant samples using capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). The control method is western blotting, where the anti-GFP antibodies enable monitoring the expression level of AHP5-GFP in individual organisms. It was found that CE-LIF is sensitive enough for both proteins. In the future, the developed methods should be used to analyze the phosphorylated and unphosphorylated AHP5-GFP. |
| Related projects: |