Analysis of GFP and AHP5-GFP Fusion Protein in the Plant Extract Using CE-LIF

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Authors

MICHALCOVÁ Lenka DOPITOVÁ Radka HEJÁTKO Jan VÁLKOVÁ Martina PREISLER Jan

Year of publication 2012
Type Article in Proceedings
Conference CECE 2012 9th International Interdisciplinary Meeting on Bioanalysis
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://www.ce-ce.org/cece2012/Proceedings%20CECE%202012%20Final+late%20abstracts_web.pdf
Field Biochemistry
Keywords CE-LIF; GF; fusion protein AHP5-GFP
Description We focused on the possibility of determination green fluorescent protein (GFP) and Arabidopsis histidine phophotransfer protein 5 fused with GFP (AHP5-GFP) in complex plant samples using capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). The control method is western blotting, where the anti-GFP antibodies enable monitoring the expression level of AHP5-GFP in individual organisms. It was found that CE-LIF is sensitive enough for both proteins. In the future, the developed methods should be used to analyze the phosphorylated and unphosphorylated AHP5-GFP.
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