Crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase from Aspergillus niger

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Authors	DEMO Gabriel FLIEDROVA Barbora WEIGNEROVA Lenka WIMMEROVÁ Michaela
Year of publication	2013
Type	Conference abstract
MU Faculty or unit	Central European Institute of Technology
Citation
Description	We report the crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase overexpressed in Pichia pastoris. The best crystals were produced by further optimization using the hanging-drop vapour diffusion method. Diffraction data were collected at BESSY II Berlin (14.1 and 14.2). The data were processed by XDSAPP. The crystals belonged to space group P1. The beta-mannosidase in the native data set diffracted to 2.41 A resolution. Experimental phasing, model fitting and refinement are in progress.
Related projects:	Enzymy metabolismu mannosidů v přípravě N-acetyl-mannosaminových struktur CEITEC - Central European Institute of Technology