Immobilization of Haloalkane Dehalogenase LinB from Sphingobium japonicum UT26 for Biotechnological Applications
| Authors | |
|---|---|
| Year of publication | 2013 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Biocatalysis & Biotransformation |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | Haloalkane Dehalogenase |
| Description | Haloalkane dehalogenases are enzymes capable of converting a broad range of aliphatic halogenated compounds to corresponding alcohols. These dehalogenase-based biotransformations are attractive for various biological processes, e.g. biocatalysis, bioremediation and detoxification, which often require protein immobilization. Different immobilization techniques, including (i) cross-linking using glutaraldehyde, dextran polyaldehyde, disuccinimidyl suberate and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, (ii) formation of cross-linked enzyme aggregates, and (iii) entrapment methods using organically modified sol-gel ORMOCER and polyvinyl alcohol particles, were systematically investigated for immobilization of selected haloalkane dehalogenase LinB from Sphingobium japonicum UT26. |
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