Dynamic Structures of DNA Heptamers with Different Central Trinucleotide Sequences Studied by Electrochemical and Spectral Methods

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Authors	PILAŘOVÁ Iveta KEJNOVSKÁ Iva VORLÍČKOVÁ Michaela TRNKOVÁ Libuše
Year of publication	2014
Type	Article in Periodical
Magazine / Source	Electroanalysis
MU Faculty or unit	Faculty of Science
Citation
Web	http://onlinelibrary.wiley.com/doi/10.1002/elan.201400288
Doi	http://dx.doi.org/10.1002/elan.201400288
Field	Physical chemistry and theoretical chemistry
Keywords	DNA heptamers; Voltammetry; Elimination voltammetric procedure; Circular dichroism; Polyacrylamide gel electrophoresis
Description	A short, single-stranded DNA fragment d(GCGAAGC) and its analogs d(GCNNNGC) carrying different trinucleotide sequences in the center of the molecule (AAA,CCC, GGG or TTT) were investigated by spectral (CD and UV spectra) and electrochemical methods (voltammetry, polyacrylamide gel electrophoresis). Our results show that (i) the central triplet GAA or AAA dramatically stabilizes DNA hairpins, (ii) a stem-loop configuration is not supported by CCC or TTT sequences, instead, these heptamers adopt bimolecular duplex forms. Finally (iii), in the case of GGG sequence a very stable supramolecular G-quadruplex is formed.
Related projects:	CEITEC - Central European Institute of Technology Chemická a elektrochemická analýza purinových derivátů Vývoj metod a instrumentace pro analýzu biologicky významných látek 2014