Determination of pyruvate and lactate as potential biomarkers of embryo viability in assisted reproduction by capillary electrophoresis with contactless conductivity detection

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Authors

MÁDR Aleš CELÁ Andrea KLEJDUS Bořivoj PELCOVÁ Marta CRHA Igor ŽÁKOVÁ Jana GLATZ Zdeněk

Year of publication 2015
Type Article in Periodical
Magazine / Source Electrophoresis
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1002/elps.201400487
Field Biochemistry
Keywords assisted reproduction; capillary electrophoresis; conductivity detection; lactate; pyruvate
Description Importance of human assisted reproduction is rising as a response for rising number of couples suffering from infertility. The key steps in human assisted reproduction are in vitro fertilization of an oocyte, cultivation of embryo and selection of an embryo with the highest developmental potential for implantation. Assessment of developmental potential of embryo is lacking of precision thus novel techniques are sought. Capillary electrophoresis with contactless conductivity detection was applied for analysis of pyruvate and lactate as potential biomarkers of developmental potential of human embryos. Optimizations of separation conditions and treatment of a sample are described and discussed. Developed method was successfully applied for analysis of several samples of spent culture media collected after cultivation of embryos.
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