Targeted-random mutagenesis of PA-IIL lectin and high-throughput screening of mutant library

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Authors	MRÁZKOVÁ Jana POKORNÁ Martina WIMMEROVÁ Michaela
Year of publication	2014
Type	Conference abstract
MU Faculty or unit	Faculty of Science
Citation
Description	PA-IIL lectin from Pseudomonas aeruginosa shows a unique binding mode among bacterial lectins because it binds saccharides through two calcium cations. Its features make this protein a good candidate for tuning its affinity and specificity. Two amino acids responsible for sugar binding were chosen for targeted-random mutagenesis. If this approach is used, it is necessary to utilize high-throughput screening of mutant libraries for identification of mutants with interestingly modified specificity and/or affinity.
Related projects:	CEITEC - Central European Institute of Technology Studium proteinu z patogenu zapojených do rozpoznávání hostitelského organismu