Reprogramming of somatic cells: possible methods to derive safe, clinical-grade human induced pluripotent stem cells

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Authors

AUGUSTYNIAK Justyna ZYCHOWICZ Marzena PODOBINSKA Martyna BÁRTA Tomáš BUZANSKA Leonora

Year of publication 2014
Type Article in Periodical
Magazine / Source Acta Neurobiologiae Experimentalis
MU Faculty or unit

Faculty of Medicine

Citation
Field Genetics and molecular biology
Keywords IPS cells; MRNA and miRNA transfection; Protein transduction; Somatic cells reprogramming
Description Derivation of pluripotent stem cells from adult somatic tissues by reprogramming technology has opened new therapeutic possibilities. Current most efficient procedures for derivation of induced pluripotent stem (iPS) cells are based on the viral vectors, which represent the danger of insertional mutagenesis during incorporation of introduced genes into the host genome. To circumvent this problem, the new, safe, non-integrative and non-viral strategies of reprogramming have been developed. In this review we discuss novel DNA-free and viral-free methods of reprogramming to iPS cells including protein transduction, mRNA and microRNA delivery.
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