THE INFLUENCE OF VERTEX POTENTIAL AND MULTIPLE SCAN VOLTAMMETRY ON THE FORMATION OF 8-OXOGUANINE

Warning

This publication doesn't include Faculty of Education. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

GURECKÝ Libor PILAŘOVÁ Iveta TRNKOVÁ Libuše

Year of publication 2015
Type Article in Proceedings
Conference XV. Workshop of Physical Chemists and Electrochemists
MU Faculty or unit

Faculty of Science

Citation
Field Physical chemistry and theoretical chemistry
Keywords guanosine; guanosine - 5 - monophosphate; PeGE; cyclic voltammetry; EVP
Description The electrochemical behavior of guanosine (Guo) and guanosine-5´-monophosphate (GMP) at pencil graphite electrode (PeGE) in the phosphate-acetic buffer is examined by cyclic voltammetry (CV). The oxidation peaks of both compounds appear at 1.1 V naturally, but using a multiple cycle we observed an additional peak. It was found that this peak corresponds to oxidation of 8-oxo-7,8-dihydroguanine (8-oG). To determine the potential of 8-oG formation we changed positive vertex potentials in the range from 0.9 to 1.4 V vs. Ag/AgCl/3MKCl reference electrode. The lowest vertex potential at which the 8-oG provided response corresponds to 1.3V for both Guo and GMP (pH 4.16). The most important is the fact that the appearance of the 8-oG peak always occurs after the first cycle of CV.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.