RECQ4 selectively recognizes Holliday junctions

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Authors

SEDLÁČKOVÁ Hana CECHOVA Barbora MLČOUŠKOVÁ Jarmila KREJČÍ Lumír

Year of publication 2015
Type Article in Periodical
Magazine / Source DNA Repair
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1016/j.dnarep.2015.02.020
Field Genetics and molecular biology
Keywords Genomic stability; Homologous recombination; RECQ4; DNA binding; Holliday junction
Description The RECQ4 protein belongs to the RecQ helicase family, which plays crucial roles in genome maintenance. Mutations in the RECQ4 gene are associated with three insidious hereditary disorders: Rothmund Thomson, Baller-Gerold, and RAPADILINO syndromes. These syndromes are characterized by growth deficiency, radial ray defects, red rashes, and higher predisposition to malignancy, especially osteosarcomas. Within the RecQ family, RECQ4 is the least characterized, and its role in DNA replication and repair remains unknown. We have identified several DNA binding sites within RECQ4. Two are located at the N-terminus and one is located within the conserved helicase domain. N-terminal domains probably cooperate with one another and promote the strong annealing activity of RECQ4. Surprisingly, the region spanning 322-400 aa shows a very high affinity for branched DNA substrates, especially Holliday junctions. This study demonstrates biochemical activities of RECQ4 that could be involved in genome maintenance and suggest its possible role in processing replication and recombination intermediates. (C) 2015 Elsevier B.V. All rights reserved.
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