Toward Structured Macroporous Hydrogel Composites: Electron Beam-Initiated Polymerization of Layered Cryogels

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Authors

GOLUNOVA Anna CHVÁTIL David KRIST Pavel JAROŠ Josef JURTÍKOVÁ Veronika POSPÍŠIL Jakub KOTELNIKOV Ilya ABELOVÁ Lucie KOTEK Jiří SEDLAČÍK Tomáš KUČKA Jan KOUBKOVÁ Jana STUDENOVSKÁ Hana STREIT Libor HAMPL Aleš RYPÁČEK František PROKS Vladimír

Year of publication 2015
Type Article in Periodical
Magazine / Source Biomacromolecules
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1021/bm501809t
Field Biochemistry
Keywords STEM-CELLS; POLYSACCHARIDE HYDROGELS; MECHANICAL-PROPERTIES; EXTRACELLULAR-MATRIX; TISSUE REGENERATION; AQUEOUS-SOLUTIONS; ADIPOSE-TISSUE; DRUG-DELIVERY; DIFFERENTIATION; SCAFFOLDS
Description The ability to tailor mechanical properties and architecture is crucial in creating macroporous hydrogel scaffolds for tissue engineering. In the present work, a technique for the modification of the pore size and stiffness of acrylamide-based cryogels is demonstrated via the regulation of an electron beam irradiation dose. The samples were characterized by equilibrium swelling measurements, light and scanning electron microscopy, mercury porosimetry, Brunauer-Emmett-Teller surface area analysis, and stiffness measurements. Their properties were compared to cryogels prepared by a standard redox-initiated radical polymerization. A I-125 radiolabeled azidopentanoyl-GGGRGDSGGGY-NH2 peptide was bound to the surface to determine the concentration of the adhesive sites available for biomimetic modification. The functionality of the prepared substrates was evaluated by in vitro cultivation of adipose-derived stem cells. Moreover, the feasibility of preparing layered cryogels was demonstrated. This may be the key to the future preparation of complex hydrogel-based scaffolds to mimic the extracellular microenvironment in a wide range of applications.
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