Method development for baseline separation of cyanobenz[f]isoindoles of proteinogenic amino acids by CE-LIF

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Authors

MÁDR Aleš CELÁ Andrea DĚDOVÁ Tereza PELCOVÁ Marta ŽÁKOVÁ Jana CRHA Igor GLATZ Zdeněk

Year of publication 2015
Type Conference abstract
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Description Monitoring of amino acids is important to understand biological processes. However, one of the most sensitive analyses employing laser-induced fluorescence detection is hindered by the fact that the most amino acids do not possess intrinsic fluorescence. As a result, amino acids were derivatized with naphthalene-2,3-dicarboxaldehyde and sodium cyanide. Method development for the baseline separation of the fluorescent products is described.
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