Způsob absolutní kvantifikace exprese miRNA, zejména miR-34a a/nebo miR-150, a jeho použití v diagnostice a prognostice B-buněčných malignit
| Title in English | Absolute quantification method of miRNA expression, particularly that of miR-34a and/or miR-150 and their use in diagnostics and prognostics of B-cell malignancies |
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| Authors | |
| Year of publication | 2016 |
| Type | Patent |
| MU Faculty or unit | |
| Publisher | Úřad průmyslového vlastnictví |
| State | Czech Republic |
| Patent's number | 306080 |
| Web | https://isdv.upv.cz/webapp/webapp.pts.det?xprim=10098590&lan=cs&s_majs=&s_puvo=&s_naze=&s_anot= |
| Description | The present invention provides an absolute quantification method of miRNA expression, being preferably selected from miR-34a and/or miR-150, wherein miRNA expression, being preferably selected from miR-34a and/or miR-150, along with the expression of endogenous check, which is a small nucleic RNA or permanently expressed miRNA are determined in a biological sample being withdrawn from a patient body. At the same time, there is determined expression of synthetic standards of miRNA, being preferably selected from miR-34a and/or miR-150 and endogenous check of known number of molecules. The invention further provides the use of the above-indicated quantification method for in vitro diagnostics of B-cell malignancies, prognostics of B-cell malignancies and prediction of therapeutic response of patients with B-cell malignancies, especially CLL, identification of TP53 abnormalities. |
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