Polymorphisms in genes encoding purinoreceptor and osteoprotegerin and external apical root resorption in children after orthodontic treatment
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| Year of publication | 2016 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
| Citation | |
| Description | Orthodontically induced external apical root resoption (EARR) is a multifactorial inflammatory disease. Genes encoding proteins, which are involved in the modulation of inflammatory response and bone remodeling, are considered as the “candidate” for EARR. The aim of this study was to analyze possible associations of single nucleotide polymorphisms (SNPs) in the P2RX7 (encoding purinoreceptor) and TNFRSF11B (encoding osteoprotegerin) genes with EARR in Czech children after orthodontic treatment. A total, 99 orthodontically treated patients (69 healthy and 30 with EARR) were enrolled in this case-control study. Genotype determination of P2RX7 +489C/T (rs208294, Tyr155His) and +1068C/T (rs1718119, Thr348Ala), TNFRSF11B -163C/T (rs3102735) and TNFRSF11B +1181C/G (rs2073618, Lys3Asn) was based on polymerase chain reaction using 5 nuclease TaqMan® assays. While no significant differences were observed in allele or genotype frequencies of all four studied SNPs, specific combinations of variants in P2RX7 may be associated with lower/higher risk of EARR development (P<0.05). In addition, the length of treatment by orthodontic appliances positively correlated with the presence of EARR (P<0.05). Although the effect of P2RX7 SNPs themselves to the development EARR was not confirmed in the Czech population, haplotype analysis suggests, that variability in the P2RX7 gene, as well as the length of treatment, may be important factors contributing to the etiopathogenesis of post-orthodontic EARR. |
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