Chromosome identification for the carnivorous plant Genlisea margaretae

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Authors	TRAN T.D. SIMKOVA H. SCHMIDT R. DOLEZEL J. SCHUBERT Ingo FUCHS J.
Year of publication	2017
Type	Article in Periodical
Magazine / Source	Chromosoma
MU Faculty or unit	Central European Institute of Technology
Citation
Web	https://link.springer.com/article/10.1007%2Fs00412-016-0599-0
Doi	http://dx.doi.org/10.1007/s00412-016-0599-0
Keywords	Genlisea; Multicolor fluorescence in situ hybridization (mcFISH); Reprobing; BACs; Karyotyping
Description	Genlisea margaretae, subgenus Genlisea, section Recurvatae (184 Mbp/1C), belongs to a plant genus with a 25-fold genome size difference and an extreme genome plasticity. Its 19 chromosome pairs could be distinguished individually by an approach combining optimized probe pooling and consecutive rounds of multicolor fluorescence in situ hybridization (mcFISH) with bacterial artificial chromosomes (BACs) selected for repeat-free inserts. Fifty-one BACs were assigned to 18 chromosome pairs. They provide a tool for future assignment of genomic sequence contigs to distinct chromosomes as well as for identification of homeologous chromosome regions in other species of the carnivorous Lentibulariaceae family, and potentially of chromosome rearrangements, in cases where more than one BAC per chromosome pair was identified.
Related projects:	EVOGEN - Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace