Analysis of microorganisms, chlorinated hydrocarbons and hyaluronic acid gel using Raman based optofluidic techniques and SERS

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Authors	SAMEK Ota PILAT Zdenek BERNATOVÁ Silvie JEZEK Jan KLEMENTOVA Tereza KIZOVSKÝ Martin ZEMANEK Pavel REBROŠOVÁ Katarína RŮŽIČKA Filip
Year of publication	2019
Type	Article in Proceedings
Conference	SPIE BiOS, 2019, San Francisco, California, United States
MU Faculty or unit	Faculty of Medicine
Citation
Doi	http://dx.doi.org/10.1117/12.2508425
Keywords	Raman spectroscopy - SERS
Description	We report on the development of a set of Raman based techniques to monitor a large variety of biological and chemical analytes, such as various microorganisms, gels of hyaluronic acid and selected halogenated hydrocarbons using Raman spectroscopy, Raman tweezers and surface-enhanced Raman spectroscopy (SERS). We analyzed individual microbial cells with Raman tweezers to provide solutions for fast and label-free identification of specific bacterial or yeast species. We designed an optofluidic SERS platform for quantification of sub-millimolar concentrations of halogenated environmental pollutants such as 1,2,3-trichloropropane and chloroform. We also examined the gel structure of hyaluronic acid by Raman spectroscopy.
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