The H3 histone chaperone NASP(SIM3) escorts CenH3 in Arabidopsis

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Authors	LE GOFF S. KECELI B.N. JERABKOVA H. HECKMANN S. RUTTEN T. COTTERELL S. SCHUBERT V. ROITINGER E. MECHTLER E. FRANKLIN F.C.H. TATOUT C. HOUBEN A. GEELEN D. PROBST A.V. LERMONTOVA Inna
Year of publication	2020
Type	Article in Periodical
Magazine / Source	Plant Journal
MU Faculty or unit	Central European Institute of Technology
Citation
Web	https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/tpj.14518
Doi	http://dx.doi.org/10.1111/tpj.14518
Keywords	Arabidopsis thaliana; centromere; kinetochore; CenH3; histone chaperone; NASP(SIM3)
Description	Centromeres define the chromosomal position where kinetochores form to link the chromosome to microtubules during mitosis and meiosis. Centromere identity is determined by incorporation of a specific histone H3 variant termed CenH3. As for other histones, escort and deposition of CenH3 must be ensured by histone chaperones, which handle the non-nucleosomal CenH3 pool and replenish CenH3 chromatin in dividing cells. Here, we show that the Arabidopsis orthologue of the mammalian NUCLEAR AUTOANTIGENIC SPERM PROTEIN (NASP) and Schizosaccharomyces pombe histone chaperone Sim3 is a soluble nuclear protein that binds the histone variant CenH3 and affects its abundance at the centromeres. NASP(SIM3) is co-expressed with Arabidopsis CenH3 in dividing cells and binds directly to both the N-terminal tail and the histone fold domain of non-nucleosomal CenH3. Reduced NASP(SIM3) expression negatively affects CenH3 deposition, identifying NASP(SIM3) as a CenH3 histone chaperone.
Related projects:	MSCAfellow@MUNI