Integration of Cryo-EM Model Building Software in Scipion

Warning

This publication doesn't include Faculty of Education. It includes Institute of Computer Science. Official publication website can be found on muni.cz.
Authors

MARTÍNEZ Marta JIMÉNEZ-MORENO Amaya MALUENDA David RAMÍREZ-APORTELA Erney MELERO Roberto CUERVO Ana CONESA Pablo DEL CANO Laura FONSECA Yunior SÁNCHEZ-GARCÍA Ruben STŘELÁK David CONESA Javier FERNÁNDEZ-GIMÉNEZ Estrella DE ISIDRO Federico SORZANO Carlos CARAZO Jose Maria MARABINI Roberto

Year of publication 2020
Type Article in Periodical
Magazine / Source Journal of Chemical Information and Modeling
MU Faculty or unit

Institute of Computer Science

Citation
Web http://dx.doi.org/10.1021/acs.jcim.9b01032
Doi http://dx.doi.org/10.1021/acs.jcim.9b01032
Keywords ELECTRON-MICROSCOPY; LOCAL RESOLUTION; VALIDATION TOOLS; VISUALIZATION; MAPS
Description Advances in cryo-electron microscopy (cryo-EM) have made it possible to obtain structures of large biological macromolecules at near-atomic resolution. This "resolution revolution" has encouraged the use and development of modeling tools able to produce high-quality atomic models from cryo-EM density maps. Unfortunately, many practical problems appear when combining different packages in the same processing workflow, which make difficult the use of these tools by non-experts and, therefore, reduce their utility. We present here a major extension of the image processing framework Scipion that provides inter-package integration in the model building area and full tracking of the complete workflow, from image processing to structure validation.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.