In-cell NMR Spectroscopy of Nucleic Acids

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Authors	DŽATKO Šimon FIALA Radovan HANSEL-HERTSCH R. TRANTÍRKOVÁ Silvie TRANTÍREK Lukáš
Year of publication	2020
Type	Article in Periodical
Magazine / Source	IN-CELL NMR SPECTROSCOPY: FROM MOLECULAR SCIENCES TO CELL BIOLOGY
MU Faculty or unit	Central European Institute of Technology
Citation
Web	https://pubs.rsc.org/en/content/chapter/bk9781788012171-00272/978-1-78801-217-1
Doi	http://dx.doi.org/10.1039/9781788013079
Keywords	DNA; guanine quadruplex; nucleic acid; water
Description	The structure, dynamics, and interactions of nucleic acids, in particular DNAs, sensitively respond to a broad range of environmental factors, many of them being natively present in the intracellular space. In-cell NMR is a tailored adaptation of conventional high-resolution nuclear magnetic resonance spectroscopy that permits characterization of stereochemical features of nucleic acids directly in living cells. Here we review recent advances and applications of in-cell NMR to nucleic acids. The limitations of this method as well as the general considerations, comprising of critical factors to be accounted for during the planning of in-cell NMR experiments, as well as during the interpretation of in-cell NMR data, are discussed.
Related projects:	Atomically resolved in vivo NMR spectroscopy as a novel tool for biological testing of therapeutically important genomic non-canonical DNA targets and their interactions with drugs in phenotypically diversified cancer cells.