Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls

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Authors	RAŠKA Jan KLÍMOVÁ Hana SHEARDOVÁ Kateřina FEDOROVÁ Veronika HŘÍBKOVÁ Hana POSPÍŠILOVÁ Veronika VOCHYÁNOVÁ Simona VÁŇOVÁ Tereza BOHAČIAKOVÁ Dáša
Year of publication	2021
Type	Article in Periodical
Magazine / Source	Stem cell research
MU Faculty or unit	Faculty of Medicine
Citation
Web	https://www.sciencedirect.com/science/article/pii/S1873506121002245?via%3Dihub
Doi	http://dx.doi.org/10.1016/j.scr.2021.102378
Keywords	human iPSC lines; spontaneous late-onset Alzheimer’s disease
Description	Human induced pluripotent stem cell (iPSC) lines were generated from patients with spontaneous late-onset Alzheimer's disease (AD) and three healthy control individuals. Peripheral blood mononuclear cells were reprogrammed with Yamanaka factors (OSKM) using a commercially available Epi5 Reprogramming Kit. The pluripotency of iPSCs was confirmed by the expression of pluripotency factors and by their ability to differentiate to all three germ layers in vitro. Newly derived cell lines can be used to model Alzheimer's disease in vitro.
Related projects:	Klinicky relevantní biochemické, imunologické a buněčné biomarkery Alzheimerovy nemoci a stárnutí Career Restart - Stem Cell-Based Models of Neurodegeneration