Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption.

Investor logo

Warning

This publication doesn't include Faculty of Education. It includes Central European Institute of Technology. Official publication website can be found on muni.cz.
Authors

KABELKA Ivo GEORGIEV Vasil MARX Lisa PAJTINKA Peter LOHNER Karl PABST Georg DIMOVA Rumiana VÁCHA Robert

Year of publication 2022
Type Article in Periodical
Magazine / Source Biophysical Journal
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://www.sciencedirect.com/science/article/pii/S000634952103959X?via%3Dihub
Doi http://dx.doi.org/10.1016/j.bpj.2021.12.035
Keywords Cell Membrane; Cryoelectron Microscopy; Lipid Bilayers; Magainins; Membrane Fusion
Description We previously speculated that the synergistically enhanced antimicrobial activity of Magainin 2 and PGLa is related to membrane adhesion, fusion, and further membrane remodeling. Here we combined computer simulations with time-resolved in vitro fluorescence microscopy, cryoelectron microscopy, and small-angle X-ray scattering to interrogate such morphological and topological changes of vesicles at nanoscopic and microscopic length scales in real time. Coarse grained simulations revealed formation of an elongated and bent fusion zone between vesicles in the presence of equimolar peptide mixtures. Vesicle adhesion and fusion were observed to occur within a few seconds by cryoelectron microscopy and corroborated by small-angle X-ray scattering measurements. The latter experiments indicated continued and time-extended structural remodeling for individual peptides or chemically linked peptide heterodimers but with different kinetics. Fluorescence microscopy further captured peptide-dependent adhesion, fusion, and occasional bursting of giant unilamellar vesicles a few seconds after peptide addition. The synergistic interactions between the peptides shorten the time response of vesicles and enhance membrane fusogenic and disruption properties of the equimolar mixture compared with the individual peptides.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.