Asociace tkáňové exprese miR-196a s Barrettovým jícnem a adenokarcinomem jícnu

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Title in English Association of tissue expression of miR-196a with Barrett's esophagus and esophageal adenocarcinoma
Authors

MLČŮCHOVÁ Natálie VYKLICKÁ Kateřina RUČKOVÁ Michaela ŠEVČÍKOVÁ Sabina BÖHM Jan VLACHOVÁ Monika DOLINA Jiří KUNOVSKÝ Lumír KROUPA Radek PAVLOVSKÝ Zdeněk DEISSOVÁ Tereza IZAKOVIČOVÁ HOLLÁ Lydie SLABÝ Ondřej KALA Zdeněk BOŘILOVÁ LINHARTOVÁ Petra

Year of publication 2023
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description Esophageal adenocarcinoma (EAC) is a highly aggressive form of cancer that often develops from Barrett's esophagus (BE), a premalignant pathological change in the lower esophagus. Specific microRNAs (miRNAs), small non-coding RNAs that function as post-transcriptional regulators of gene expression, have been repeatedly shown to play a key role in the pathogenesis of these diseases. This pilot study aimed to analyze four selected miRNAs in esophageal tissue of healthy controls (HC) and patients with reflux esophagitis (RE)/BE/EAC. Another aim was to compare expression in Barrett's esophagus/adenocarcinoma tissue and in tissue outside the main pathology in BE/EAC patients. Twenty-two subjects (3 HC, 8 RE, 5 BE, 6 EAC) who underwent endoscopic examination were included in the study. RNA was isolated from frozen esophageal tissue samples (preserved in RNAlater™ Stabilization Solution at -70°C) using the AllPrep DNA/RNA/miRNA Universal Kit. Subsequent RT-qPCR analysis was performed using selected TaqMan MicroRNA Assays for miR21, miR34a, miR196a, miR196b and endogenous controls (RNU44).? While miR-21 expression in pathological esophageal tissue was decreased in BE and EAC patients compared to HC and RE patient groups (p=0.01), miR-196a expression was increased in BE and EAC patients (p<0.01). A correlation was observed between the expression of these two miRNAs and the severity of diagnosis (p<0.05). In addition, we observed that miR-196a was significantly more expressed at the site with the main pathology compared to paired adjacent esophageal tissue in BE and EAC patients (p<0.01). In previous studies, miR-196a has been associated with esophageal squamous cell carcinoma and is therefore viewed as a potential therapeutic target in this disease. Consistent with the studies by Luzno et al. and Maru et al, the results of our pilot study suggest that miR-196a, which regulates cell proliferation, invasion and migration, could become a suitable diagnostic tissue biomarker for BE and EAC.
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