Generation of the Human iPSC Line from Spontaneous Late-Onset Alzheimer's Disease Patient with ApoE3/3 Genotype and Sex-, Age-, and ApoE-Matched Healthy Control

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Authors

KADÁKOVÁ Soňa RAŠKA Jan HŘÍBKOVÁ Hana FEDOROVÁ Veronika SEDMÍK Jiří PLEŠINGROVÁ Klára SHEARDOVÁ Kateřina BOHAČIAKOVÁ Dáša

Year of publication 2024
Type Article in Periodical
Magazine / Source Stem cell research
MU Faculty or unit

Faculty of Medicine

Citation
Web https://www.sciencedirect.com/science/article/pii/S1873506123002593?via%3Dihub
Doi http://dx.doi.org/10.1016/j.scr.2023.103273
Keywords Human iPSC Line; Alzheimer’s Disease; ApoE3/3 Genotype
Attached files
Description Human induced pluripotent stem cell (iPSC) lines were generated from peripheral blood mononuclear cells (PBMCs) isolated from a patient diagnosed with spontaneous late -onset Alzheimer's disease (AD) carrying ApoE3/3 gene and one age-, sex-, and ApoE-matched healthy control. Reprogramming was done using a commercially available Epi5 Reprogramming Kit containing OCT4, SOX2, KLF4, LIN28, and L-MYC as reprogramming factors. The pluripotency of the iPSC lines was verified by the expression of pluripotency markers and by their capacity to differentiate into all three embryonic germ layers in vitro. These newly established iPSC lines offer a valuable platform for in vitro modeling of AD.
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