Nuclear topography of the c-myc gene in human leukemic cells

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Authors	BÁRTOVÁ Eva KOZUBEK Stanislav KOZUBEK Michal JIRSOVÁ Pavla LUKÁŠOVÁ Emilie SKALNÍKOVÁ Magdalena CAFOURKOVÁ Alena KOUTNÁ Irena
Year of publication	2000
Type	Article in Periodical
Magazine / Source	Gene
MU Faculty or unit	Faculty of Informatics
Citation
Web	abstract
Field	Genetics and molecular biology
Keywords	c-myc topography; differentiation; FISH; HL-60; U-937
Description	myc gene plays an essential role in the regulation of the cell cycle and differentiation. Therefore, changes of the c-myc positioning during differentiation are of great interest. As a model system of cell differentiation, the HL-60 and U-937 human leukemic cell lines, were used in our experiments. These cells can be induced to differentiation into granulocytes that represent one of the pathways of blood cell maturation. In this study, changes of the topographic characteristics of the c-myc gene (8q24), centromeric region of chromosome 8 and chromosome 8 domain during differentiation of HL-60 and U-937 cells were detected using fluorescence in situ hybridisation (FISH). FISH techniques and fluorescence microscopy combined with image acquisition and analysis (High Resolution Cytometry) were used in order to detect the topographic features of nuclear chromatin.
Related projects:	Image analysis in the study of mechanisms of induction, in diagnosis and for prevention of deleterious human diseases The structure of interphase nucleus and its changes after irradiation Changes in the structure of interphase nucleai of human leukemic cell lines after treatment with differentiating agents and gama irradiation The use interphase FISH for the determination of individuals with elevated risk of the induction of hemoblastoses Application of computer image analysis in optical microscopy