Nuclear topography of the c-myc gene in human leukemic cells

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Authors

BÁRTOVÁ Eva KOZUBEK Stanislav KOZUBEK Michal JIRSOVÁ Pavla LUKÁŠOVÁ Emilie SKALNÍKOVÁ Magdalena CAFOURKOVÁ Alena KOUTNÁ Irena

Year of publication 2000
Type Article in Periodical
Magazine / Source Gene
MU Faculty or unit

Faculty of Informatics

Citation
Web abstract
Field Genetics and molecular biology
Keywords c-myc topography; differentiation; FISH; HL-60; U-937
Description myc gene plays an essential role in the regulation of the cell cycle and differentiation. Therefore, changes of the c-myc positioning during differentiation are of great interest. As a model system of cell differentiation, the HL-60 and U-937 human leukemic cell lines, were used in our experiments. These cells can be induced to differentiation into granulocytes that represent one of the pathways of blood cell maturation. In this study, changes of the topographic characteristics of the c-myc gene (8q24), centromeric region of chromosome 8 and chromosome 8 domain during differentiation of HL-60 and U-937 cells were detected using fluorescence in situ hybridisation (FISH). FISH techniques and fluorescence microscopy combined with image acquisition and analysis (High Resolution Cytometry) were used in order to detect the topographic features of nuclear chromatin.
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